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KMID : 0385520160290020079
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Analytical Science & Technology
2016 Volume.29 No. 2 p.79 ~ p.84
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Performance of MiniPCRTM mini8, a portable thermal cycler
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Kwon Han-Sol
Park Hyun-Chul
Lee Kyung-myung
An Sang-hyun
Oh Yu-Li
Ahn Eu-Ree
Jung Ju-Yeon
Lim Si-Keun
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Abstract
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A small and inexpensive thermal cycler (PCR machine), known as the MiniPCRTM Mini8 Thermal Cycler (Amplyus, Cambridge, MA, USA), was developed. In this study, the performance of this PCR machine was compared with the GeneAmp® PCR system 9700 (Applied Biosystems) using four autosomal short tandem repeat (STR) kits, a Y-chromosome STR kit, and a mitochondrial DNA HV1/HV2 sequence analysis. The sensitivity and stochastic effects of the STR multiplex kits and the quality of the DNA sequence analysis were similar between the two PCR machines. The MiniPCRTM Mini8 Thermal Cycler could be used for analyses at forensic DNA laboratories and crime scenes. The cost of the PCR is so economical that school laboratories and individuals could use the machines.
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KEYWORD
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MiniPCRTM mini8 Thermal Cycler, GeneAmp®PCR system 9700, performance, STR multiplex kits, mtDNA HV1/HV2,
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